The next monoclonal antibodies have been ordered out-of Pharmingen (North park, Calif
Phone staining and flow cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaNstep three) with optimal amounts of fluorescein isothiocyanate-, phycoerythrin-, or biotin-conjugated… Read more »